{"product_id":"himedia-bile-esculin-agar-modified-m972a-500g","title":"HiMedia - Bile Esculin Agar, Modified (M972A-500G).","description":"\u003cp\u003e\u003cspan\u003eA differential medium recommended for isolation and presumptive identification of group D Streptococci\/Enterococci from food and pharmaceutical products.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"section intended-use\"\u003e\n\u003ch3\u003eIntended use\u003c\/h3\u003e\n\u003cp\u003eRecommended for the isolation and presumptive identification of group D Streptococci\/ Enterococci from food, pharmaceutical products and clinical samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section composition\"\u003e\n\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eGms \/ Litre\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHM Peptone B #\u003c\/td\u003e\n\u003ctd\u003e3.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBile $\u003c\/td\u003e\n\u003ctd\u003e20.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEsculin\u003c\/td\u003e\n\u003ctd\u003e1.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerric citrate\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH (at 25°C)\u003c\/td\u003e\n\u003ctd\u003e7.1±0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"note\"\u003eFormula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003cp class=\"note\"\u003e# - Equivalent to Beef extract; $- Equivalent to Oxgall\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section directions\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 44.5 grams in 1000 ml purified\/distilled water. Heat to boiling to dissolve the medium completely. Mix and dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubed medium to solidify in slanted position.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section principle_and_interpretation\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eThis medium is a modification of Bile esculin agar, M972 that differs in content of bile. The value of bile tolerance together with hydrolysis of esculin as a means of presumptive identification of group D streptococci\/ enterococci is widely recognized (1). Bile esculin agar was formulated by Swan (2) for the isolation and identification of Group D Streptococci from food. This medium was also shows differentiation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacteriaceae\u003c\/i\u003e,\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eKlebsiella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacter-Serratia\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003edivision from other\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacteriaceae\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003egenera on the basis of esculin hydrolysis (3). Peptone and HM Peptone B provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. The medium contains 2% bile that inhibits gram-positive bacteria other than group D Streptococci and Enterococci. Enterococci and Group D Streptococci hydrolyzes esculin to esculetin and dextrose, which reacts with ferric citrate producing brownish black precipitate (4). Originally Bile esculin test was used for identification of Enterococci. But it was found that this test is also shared by group D Streptococci (3) and therefore it is recommended that other tests such as salt tolerance must be performed while identifying Enterococci (4).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section type_of_specimen\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical samples- faeces, Food and dairy samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section specimen_collection_and_handling\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (2,7,8). After use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section warning_and_precautions\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves\/protective clothing\/eye protection\/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section limitations\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e1. Further biochemical tests must be carried out in conjunction for confirmation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section performance_and_evaluation\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section quality_control\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003ch4\u003eAppearance\u003c\/h4\u003e\n\u003cp\u003eLight yellow to brownish yellow coloured homogeneous free flowing powder\u003c\/p\u003e\n\u003ch4\u003eGelling\u003c\/h4\u003e\n\u003cp\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003ch4\u003eColour and Clarity of prepared medium\u003c\/h4\u003e\n\u003cp\u003eYellow coloured clear to slightly opalescent gel with\/without a bluish tinge forms in Petri plates.\u003c\/p\u003e\n\u003ch4\u003eReaction\u003c\/h4\u003e\n\u003cp\u003eReaction of 4.5% w\/v aqueous solution at 25°C pH : 7.1±0.2\u003c\/p\u003e\n\u003ch4\u003epH\u003c\/h4\u003e\n\u003cp\u003e6.90-7.30\u003c\/p\u003e\n\u003ch4\u003eCultural Response\u003c\/h4\u003e\n\u003cp\u003eCultural characteristics observed after an incubation at 35°C-37°C for 18-24 hours in an increased atmosphere of carbon dioxide.\u003c\/p\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eEsculin hydrolysis\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEnterococcus faecalis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 29212 (00087*)\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003ePositive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStreptococcus pyogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 12344\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003eNegative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eKlebsiella aerogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13048 (00175*)\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003ePositive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eProteus mirabilis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25933\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003eNegative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"note\"\u003eKey: *Corresponding WDCM numbers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section storage_and_shelf_life\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section disposal\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section references\"\u003e\n\u003ch3\u003eReferences\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eFacklam R., 1968, Appl. Microbiol\u003c\/li\u003e\n\u003cli\u003eSalfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods, American Public Health Association, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eSwan A., 1954, J. Clin. Pathol., 7:160\u003c\/li\u003e\n\u003cli\u003eFacklam R., 1973, Appl. Microbiol., 26:138.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003cli\u003eAmerican Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington\u003c\/li\u003e\n\u003cli\u003eWehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M972A.pdf\" target=\"_blank\" title=\"Bile Esculin Agar, Modified\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Himedia","offers":[{"title":"Default Title","offer_id":48377357107419,"sku":"BP-5262-HIM-HIM-DEF","price":11929.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m972a-500g.png?v=1773657971","url":"https:\/\/zayyuconnect.com\/ar\/products\/himedia-bile-esculin-agar-modified-m972a-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}