{"product_id":"himedia-cetrimide-agar-base-m024-100g","title":"HiMedia - Cetrimide Agar Base (M024-100G).","description":"\u003cdiv class=\"attr-info\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eRecommended for selective isolation of Pseudomonas aeruginosa from clinical specimens.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended use\u003c\/h3\u003e\n\u003cp\u003eRecommended for the selective isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003efrom water and clinical specimens.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition**\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGelatin peptone\u003c\/td\u003e\n\u003ctd\u003e20.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium chloride\u003c\/td\u003e\n\u003ctd\u003e1.400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium sulphate\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCetrimide\u003c\/td\u003e\n\u003ctd\u003e0.300\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C):\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.2±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 46.7 grams in 1000 ml purified\/distilled water containing 10 ml glycerol. Heat, to boiling, to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired, rehydrated contents of 1 vial of Nalidixic Selective Supplement (FD130) may be added aseptically to 1000 ml medium. Mix well and pour into sterile Petri plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003egrows well on all normal laboratory media but specific isolation of the organism, from environmental sites or from human, animal or plant sources, is best carried out on a medium, which contains a selective agent and also constituents to enhance pigment production. Most selective media depend upon the intrinsic resistance of the species to various antibacterial agents. Cetrimide inhibits the growth of many microorganisms whilst allowing\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eto develop typical colonies.\u003c\/p\u003e\n\u003cp\u003eCetrimide is a quaternary ammonium salt, which acts as a cationic detergent that reduces surface tension in the point of contact and has precipitant, complexing and denaturing effects on bacterial membrane proteins. It exhibits inhibitory actions on a wide variety of microorganisms including\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies other than\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e. King et al developed Medium A for the enhancement of pyocyanin production by\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e(1). Cetrimide Agar developed by Lowburry (1) is a modification of Tech Agar (Medium A) with addition of 0.1% cetrimide for selective isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e. Later, due to the availability of the highly purified cetrimide, its concentration in the medium was decreased (2,3). The incubation was carried out at 37°C for a period of 18-24 hours (4).\u003c\/p\u003e\n\u003cp\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ecan be identified due to their characteristic production of pyocyanin, a blue, water-soluble, non-fluorescent phenazine pigment coupled with their colonial morphology and the characteristic grape-like odor of aminoacetophenone (5).\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eis the only species of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eor gram-negative rod known to excrele pyocyanin. These media are therefore, important in the identification of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e. These media are used for the examination of cosmetics (6) and clinical specimens (5,7) for the presence of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e, as well as for evaluating the efficacy of disinfectants against this organism (8).\u003c\/p\u003e\n\u003cp\u003eGelatin peptone provide necessary nutrients for\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e. Sodium chloride maintains osmotic equilibrium in the medium. Magnesium chloride and potassium sulfate stimulates pyocyanin production (9).\u003c\/p\u003e\n\u003cp\u003eFor the isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e, plates of Cetrimide Agar should be inoculated from non-selective medium such as Brain Heart Infusion Broth (M210) or Soyabean Casein Digest Medium (M011). If the count is high, the test sample can be directly inoculated onto Cetrimide Agar.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ecolonies may appear pigmented blue, blue-green or non-pigmented. Colonies exhibiting fluorescence at 250nm and a blue green pigmentation are considered as presumptive positive.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003emay lose its fluorescence under UV if the cultures are left at room temperature for a short time. Fluorescence reappears after the plates are re-incubated (4). Type of peptone used in the base may also affect pigment production (4,10).\u003c\/p\u003e\n\u003cp\u003eCertain strains of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eP.aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003emay not produce pyocyanin. Other species of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003edo not produce pyocyanin but fluoresce under UV light. Most non-\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies are inhibited on Cetrimide Agar, and some species of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003emay also be inhibited. Some non-fermenters and some aerobic spore formers may exhibit a water-soluble tan to brown pigmentation on this medium.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSerratia\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003emay exhibit pink pigmentation (3). Biochemical tests and serological procedures should be performed to confirm the findings.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical samples - urine, wound ; Water samples\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling:\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (11,12).\u003c\/p\u003e\n\u003cp\u003eFor water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards(13).\u003c\/p\u003e\n\u003cp\u003eAfter use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions :\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves\/ protective clothing\/eye protection\/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations :\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eThis medium is a selective medium, some strains may show poor growth as cetrimide is highly toxic.\u003c\/li\u003e\n\u003cli\u003eFurther biochemical tests must be carried out for further confirmation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eLight amber coloured opalescent gel with a slight precipitate forms in Petri plates\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 4.67% w\/v aqueous solution containing 1% glycerol at 25°C. pH: 7.2±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.00-7.40\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCultural response\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCultural response was observed after an incubation at 30-35°C for specified time. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.\u003c\/p\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eObserved Lot value (CFU)\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003cth\u003eIncubation temperature\u003c\/th\u003e\n\u003cth\u003eIncubation period\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e^\u003ci\u003ePseudomonas paraeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 9027 (00026*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e25-100\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=18 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 8739 (00012*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 27853 (00025*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e25-100\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e18-24 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25668 (00114*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e25-100\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e18-24 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStenotrophomonas maltophila\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13637\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.aureus ATCC 6538 (00032*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.aureus ATCC 25923 (00034*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Typhimurium\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 14028 (00031*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eProteus mirabilis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 29906 (00023*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"note\"\u003eKey: *Corresponding WDCM numbers, ^ Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (11,12).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eKing, Ward and Raney, 1954, J. Lab. Clin. Med., 44:301.\u003c\/li\u003e\n\u003cli\u003eLowbury, 1951, J. Clin. Pathol., 4:66.\u003c\/li\u003e\n\u003cli\u003eLowbury and Collins, 1955, J. Clin. Pathol., 8:47\u003c\/li\u003e\n\u003cli\u003eBrown and Lowbury, 1965, J. Clin. Pathol., 18:752.\u003c\/li\u003e\n\u003cli\u003eMurray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and YolkenR. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eUSFDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.\u003c\/li\u003e\n\u003cli\u003eForbes B. A., Sahm A. S. and Weissfeld D. F., Bailey \u0026amp; Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc., St. Louis, Mo.\u003c\/li\u003e\n\u003cli\u003eWilliams, (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th Ed., AOAC, Washington, D.C\u003c\/li\u003e\n\u003cli\u003eMacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification -Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.\u003c\/li\u003e\n\u003cli\u003eGoto and Enomoto, 1970, Jpn. J. Microbiol., 14:65.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003cli\u003eLipps WC, Braun-Howland EB, Baxter TE, eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M024.pdf\" target=\"_blank\" title=\"Cetrimide Agar Base\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himedia","offers":[{"title":"Default Title","offer_id":48765165535451,"sku":"BP-5335-HIM-HIM-DEF","price":1299.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m024-100g.png?v=1778654001","url":"https:\/\/zayyuconnect.com\/ar\/products\/himedia-cetrimide-agar-base-m024-100g","provider":"Zayyu Connect","version":"1.0","type":"link"}