{"product_id":"himedia-fluid-thioglycollate-medium-thioglycollate-medium-fluid-m009-100g","title":"HiMedia - Fluid Thioglycollate Medium (Thioglycollate Medium, Fluid) (M009-100G).","description":"\u003cdiv class=\"attr-info\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eRecommended for sterility testing of biologicals and for cultivation of aerobes, anaerobes and microaerophiles.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended Use\u003c\/h3\u003e\n\u003cp\u003eRecommended for sterility testing of biologicals and for cultivation of anaerobes, aerobes and microaerophiles from pharmaceutical and clinical samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDextrose (Glucose)\u003c\/td\u003e\n\u003ctd\u003e5.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd\u003e2.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Cystine\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium thioglycollate\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResazurin sodium\u003c\/td\u003e\n\u003ctd\u003e0.001\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e0.750\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C):\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.1±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 29.75 grams in 1000 ml purified\/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C and store in a cool dark place preferably below 25°C.\u003c\/p\u003e\n\u003cp class=\"note\"\u003e\u003cb\u003eNote:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eIf more than the upper one-third of the medium has acquired a pink-purple colour, the medium may be restored once by heating in a water bath or in free flowing steam until the pink-purple colour disappears.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eBrewer (1) formulated Fluid Thioglycollate Medium for rapid cultivation of aerobes as well as anaerobes including microaerophiles by adding a reducing agent and small amount of agar. The BP (2), EP (3), USP (4), and AOAC (5) have recommended the media for sterility testing of antibiotics, biologicals and foods and for determining the phenol coefficient and sporicidal effect of disinfectants. However, it is intended for the examination of clear liquid or water-soluble materials. Fluid Thioglycollate Medium is also routinely used to check the sterility of stored blood in blood banks (6).\u003c\/p\u003e\n\u003cp\u003eDextrose, tryptone, yeast extract, L-cystine provide the growth factors necessary for bacterial multiplication. L-cystine and sodium thioglycollate allows Clostridium to grow in this medium even under aerobic conditions (7). Also the small amount of agar used in the medium favors the growth of aerobes as well as anaerobes in the medium, even if sodium thioglycollate is deleted from the medium(1). Sodium thioglycollate act as a reducing agent and neutralizes the toxic effects of mercurial preservatives and peroxides formed in the medium, thereby promoting anaerobiosis, and making the medium suitable to test materials containing heavy metals. (8,9). Any increase in the oxygen content is indicated by a colour change of redox indicator, resazurin to red (6,10,11). The small amount of agar helps in maintaining low redox potential for stabilizing the medium (9).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003ePharmaceutical samples for sterility testing, clinical samples- pus, wounds\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling:\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (12,13).\u003c\/p\u003e\n\u003cp\u003eFor pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines (2,3,4) After use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves\/protective clothing\/eye protection\/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e1. It is intended for the examination of clear liquid or water-soluble materials.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eLight straw coloured, clear to slightly opalescent solution with upper 10% or less medium pink-purple on standing.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 2.97% w\/v aqueous solution at 25°C. pH: 7.1±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e6.90-7.30\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGrowth Promotion Test:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eAs per USP\/EP\/BP\/IP\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGrowth promoting properties:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eClearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating \u0026lt;=100 cfu at 30-35°C for or not more than 3 days for aerobes and anaerobes.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eSterility Testing + Validation:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eThe medium is tested with suitable strains of microrganisms inoculating \u0026lt;=100cfu and incubating at 20-25°C for not more than 3 days in case of bacteria and not more than 5 days in case of fungi.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eTesting in accordance with EN ISO 11133:2014\/Amd.1:2018(E) (10):\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCultural characteristics observed after an incubation at 36-38°C for 18-24 hours\u003c\/p\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ch4\u003eGrowth promoting (30-35°C)\u003c\/h4\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eIncubation at\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eStaphylococcus aureus subsp. aureus ATCC 6538 (00032*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStaphylococcus aureus subsp. aureus ATCC 25923 (00034*) ^\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e$ Bacillus spizizenii ATCC 6633 (00003*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e^Pseudomonas paraeruginosa ATCC 9027 (00026*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e##Kocuria rhizophila ATCC 9341\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eClostridium sporogenes ATCC 19404 (00008*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eClostridium sporogenes ATCC 11437\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e$Phocaeicola vulgatus ATCC 8482\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEscherichia coli ATCC 8739 (00012*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSalmonella Typhimurium ATCC 14028 (00031*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSalmonella Abony NCTC 6017\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e30-35°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ch4\u003eSterility Testing- Growth promotion + Validation (20-25°C)\u003c\/h4\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eIncubation at\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eStaphylococcus aureus subsp. aureus ATCC 6538 (00032*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e20-25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e$ Bacillus spizizenii ATCC 6633 (00003*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e20-25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e^Pseudomonas paraeruginosa ATCC 9027 (00026*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e20-25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e##Kocuria rhizophila ATCC 9341\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e20-25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCandida albicans ATCC 10231 (00054*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e20-25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e#Aspergillus brasiliensis ATCC 16404 (00053*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e20-25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ch4\u003eCultural characteristics observed (36-38°C)\u003c\/h4\u003e\n\u003cp\u003eCultural characteristics observed after an incubation at 36-38°C for 18-24 hours\u003c\/p\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eIncubation at\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eClostridium perfringens ATCC 13124 (00007*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e36-38°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cb\u003eKey:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e* Corresponding WDCM numbers,\u003c\/li\u003e\n\u003cli\u003e## Formerly known as Micrococcus luteus\u003c\/li\u003e\n\u003cli\u003e$ Formerly known as Bacteroides vulgatus\u003c\/li\u003e\n\u003cli\u003e# Formerly known as Aspergillus niger,\u003c\/li\u003e\n\u003cli\u003e$ Formerly known as Bacillus subtilis subsp. spizizenii\u003c\/li\u003e\n\u003cli\u003e^ Formerly known as Pseudomonas aeruginosa\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (12,13).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eBrewer, 1940, J. Am. Med. Assoc., 115:598.\u003c\/li\u003e\n\u003cli\u003eThe British Pharmacopoeia, 2022, Medicines and Healthcare products Regulatory Agency.\u003c\/li\u003e\n\u003cli\u003eEuropean Pharmacopoeia, 2022, 10 th volume, European Directorate for the quality of medicines \u0026amp; Healthcare.\u003c\/li\u003e\n\u003cli\u003eThe United States Pharmacopoeia-National Formulatory (USP-NF), 2022\u003c\/li\u003e\n\u003cli\u003eWilliams H., (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th Ed., AOAC,Washington, D.C\u003c\/li\u003e\n\u003cli\u003eNungester, Hood and Warren, 1943, Proc. Soc. Exp. Biol. Med., 52:287.\u003c\/li\u003e\n\u003cli\u003ePortwood, 1944, J. Bact., 48:255.\u003c\/li\u003e\n\u003cli\u003eFederal Register, 1992, Fed. Regist., 21:640.\u003c\/li\u003e\n\u003cli\u003eQuastel and Stephenson, 1926, J.Biochem., 20\u003c\/li\u003e\n\u003cli\u003eMarshall, Gunnison and Luxen, 1940, Proc. Soc. Exp. Biol. Med., 43:672.\u003c\/li\u003e\n\u003cli\u003eMacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams and Wilkins, Baltimore.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M009.pdf\" target=\"_blank\" title=\"Fluid Thioglycollate Medium (Thioglycollate Medium, Fluid)\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himedia","offers":[{"title":"Default Title","offer_id":48765160685787,"sku":"BP-5333-HIM-HIM-DEF","price":6649.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m009-100g.png?v=1778653639","url":"https:\/\/zayyuconnect.com\/ar\/products\/himedia-fluid-thioglycollate-medium-thioglycollate-medium-fluid-m009-100g","provider":"Zayyu Connect","version":"1.0","type":"link"}