{"product_id":"himedia-macconkey-agar-w-0-15-bile-salts-cv-and-nacl-m081-100g","title":"HiMedia - MacConkey Agar w\/ 0.15% Bile Salts, CV and NaCl (M081-100G)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eFor the selective isolation and differentiation of coliform organisms and other enteric pathogens from clinical and non-clinical samples.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cp\u003eFor the selective isolation and differentiation of coliform organisms and other enteric pathogens from clinical and non clinical samples.\u003c\/p\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGelatin peptone\u003c\/td\u003e\n\u003ctd\u003e17.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone\u003c\/td\u003e\n\u003ctd\u003e1.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd\u003e1.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLactose\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBile salts\u003c\/td\u003e\n\u003ctd\u003e1.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeutral red\u003c\/td\u003e\n\u003ctd\u003e0.030\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCrystal violet\u003c\/td\u003e\n\u003ctd\u003e0.001\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C):\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.1±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 51.53 grams in 1000 ml purified\/distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. The surface of the medium should be dry when inoculated.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eMacConkey agars are slightly selective and differential plating media mainly used for the detection and isolation of gram-negative organisms from clinical (1), dairy (2), food (3,4), water (5), pharmaceutical (6,7) and industrial sources (8). It is also recommended for the selection and recovery of the\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacteriaceae\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand related enteric gram-negative bacilli. USP recommends this medium for use in the performance of Microbial Limit Tests (7).\u003c\/p\u003e\n\u003cp\u003eThese agar media are selective since the concentration of bile salts, which inhibit gram-positive microorganisms, is low in comparison with other enteric plating media. The medium M081, which corresponds with, that recommended by APHA can be used for the direct plating of water samples for coliform bacilli, for the examination of food samples for food poisoning organisms (4) and for the isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies in cheese (2). Other than that this medium is also used for count of coli-aerogenes bacteria in cattle and sheep faeces (9), the count of coli-aerogenes and non-lactose fermenters in poultry carcasses (9), bacterial counts on irradiated canned minced chicken (10) and the recognition of coli-aerogenes bacteria during investigations on the genus\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eAeromonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e(11,12).\u003c\/p\u003e\n\u003cp\u003eMacConkey Agar is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens (13,14). The original medium contains protein, bile salts, sodium chloride and two dyes. The selective action of this medium is attributed to crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria. Gram-negative bacteria usually grow well on the medium and are differentiated by their ability to ferment lactose. Lactose-fermenting strains grow as red or pink colonies and may be surrounded by a zone of acid precipitated bile. The red colour is due to production of acid from lactose, absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8. Lactose non-fermenting strains, such as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eare colourless, transparent and typically do not alter appearance of the medium.\u003c\/p\u003e\n\u003cp\u003ePeptone, Tryptone and gelatin peptone are sources of nitrogen, carbon, long chain amino acids and other nutrients. Lactose is a fermentable carbohydrate, Sodium chloride maintains the osmotic equilibrium. Bile salts and crystal violet are selective agents that inhibit growth of gram-positive organisms. Neutral red is the pH indicator dye.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical - faeces, urine etc., foodstuffs and dairy samples, water samples\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,15).\u003c\/p\u003e\n\u003cp\u003eFor food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (2,4).\u003c\/p\u003e\n\u003cp\u003eFor water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5).\u003c\/p\u003e\n\u003cp\u003eAfter use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves\/ protective clothing\/eye protection\/face protection. Follow good microbiological lab practices while handling clinical specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eThough the medium is recommended for selective isolation, further biochemical and serological testing must be carried out for further confirmation.\u003c\/li\u003e\n\u003cli\u003eThe surface of the medium should be dry when inoculated.\u003c\/li\u003e\n\u003cli\u003eIndividual organisms differ in their growth requirement and may show variable growth patterns on the medium.\u003c\/li\u003e\n\u003cli\u003eEach lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eLight yellow to pink homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFirm comparable with 1.5% Agar gel.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eRed with purplish tinge coloured clear to slightly opalescent gel forms in Petri plates.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 5.15% w\/v aqueous solution at 25°C. pH: 7.1±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e6.90-7.30\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eCultural Response\u003c\/h3\u003e\n\u003cp\u003eCultural response was observed after an incubation at 30-35°C for 18-72 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar.\u003c\/p\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003cth\u003eColour of colony\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ci\u003eCorynebacterium diphtheriae type gravis\u003c\/i\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eShigella flexneri\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 12022 (00126*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair to good\u003c\/td\u003e\n\u003ctd\u003e30-40%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Paratyphi\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eA ATCC 9150\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Abony\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eNCTC 6017 (00029*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e##\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eProteus hauseri\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13315\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Typhi\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 6539\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus epidermidis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 12228 (00036*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 8739 (00012*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003epink-red with bile precipitate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.aureus ATCC 6538 (00032*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Paratyphi\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eB ATCC 8759\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003epink to red with bile precipitate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e#\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eKlebsiella aerogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13048 (00175*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003epink to red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Typhimurium\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 14028 (00031*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEnterococcus faecalis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 29212 (00087*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003enone - poor\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=10%\u003c\/td\u003e\n\u003ctd\u003ecolourless to pale pink\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Enteritidis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13076 (00030*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.aureus ATCC 25923 (00034*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e4\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cb\u003eKey :-\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e* Corresponding WDCM numbers, # Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacter aerogenes\u003c\/i\u003e, ## Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eProteus vulgaris\u003c\/i\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,15).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eMurray P. R, Baron E, J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical Microbiology, ASM, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eWehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eFDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eSalfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eLipps WC, Braun-Howland EB, Baxter TE, eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.\u003c\/li\u003e\n\u003cli\u003eBritish Pharmacopoeia, 2019, The Stationery office British Pharmacopoeia.\u003c\/li\u003e\n\u003cli\u003eThe United States Pharmacopoeia, 2018, The United States Pharmacopeial Convention, Rockville, M.D.\u003c\/li\u003e\n\u003cli\u003eWilliams, (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th Ed., AOAC, Washington, D.C\u003c\/li\u003e\n\u003cli\u003eMedrek T. F and Barnes Ella M., 1962, J. Appl. Bacteriol., 25(2),159-168.\u003c\/li\u003e\n\u003cli\u003eThornley Margaret J., 1957, J. Appl. Bacteriol., 20(2), 273-285.\u003c\/li\u003e\n\u003cli\u003eBarnes Ella M. and Shrimpton D. H., 1957, J. Appl. Bacteriol., 20(2),273-285.\u003c\/li\u003e\n\u003cli\u003eEddy B. P., 1960, J. Appl. Bacteriol., 23(2).216-249.\u003c\/li\u003e\n\u003cli\u003eMacConkey A., 1905, J. Hyg., 5:333.\u003c\/li\u003e\n\u003cli\u003eMacConkey A., 1900, The Lancet, ii:20.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M081.pdf\" target=\"_blank\" title=\"MacConkey Agar w\/ 0.15% Bile Salts, CV and NaCl\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"Default Title","offer_id":48237348552923,"sku":"BP-5214-HIM-HIM-DEF","price":999.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m081-100g.png?v=1770639882","url":"https:\/\/zayyuconnect.com\/ar\/products\/himedia-macconkey-agar-w-0-15-bile-salts-cv-and-nacl-m081-100g","provider":"Zayyu Connect","version":"1.0","type":"link"}