{"product_id":"himedia-r2a-broth-m1687-500g","title":"HiMedia - R2A Broth (M1687-500G).","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eFor cultivation and maintenance of heterotrophic bacteria from potable waters.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003cp\u003eR-2A Broth is used for cultivation and maintenance of heterotrophic bacteria from potable waters.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition**\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eGms \/ Litre\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCasein acid hydrolysate\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteose peptone\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDextrose\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStarch, soluble\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium phosphate\u003c\/td\u003e\n\u003ctd\u003e0.300\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium sulphate\u003c\/td\u003e\n\u003ctd\u003e0.024\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium pyruvate\u003c\/td\u003e\n\u003ctd\u003e0.300\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH ( at 25°C)\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.2±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 3.12 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. DO NOT OVERHEAT.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eThe total bacterial count of drinking water is determined by plate count on a nutritionally rich medium. However all organisms present are not able to grow on them, either because they are slow growers or because they cant grow on that media (1). For this reason a nutritionally reduced medium was described. R-2A Agar is a modification of this medium (2,3).\u003c\/p\u003e\n\u003cp\u003eR-2A Agar is an alternative medium used for the heterotrophic plate counts and for subculturing isolates from potable waters (1). R-2A Agar is also recommended by APHA (4) for pour plate, spread plate and membrane filter technique. R-2A Broth is similar to R-2A Agar except agar. Total count recommended for the bacterial examination of potable waters gives an estimate of the aerobic and facultatively anaerobic bacteria, which grow best at 35°C in a rich medium (3). R-2A Broth enables better recovery of these bacteria from treated waters under different incubation conditions. Many bacteria from natural waters, which contain limited nutrients at ambient temperature, grow best on the media with less nutrient levels. They grow better at the temperatures below the routine laboratory incubation temperatures of 35 to 37°C (3).\u003c\/p\u003e\n\u003cp\u003eThis medium contains casein acid hydrolysate, yeast extract, biopeptone as source of essential growth factors required for metabolism of the bacteria. Dextrose is the energy source. Starch acts as a neutralizer that neutralizes any toxic metabolites, if present. Phosphate buffers the medium while sodium pyruvate supplies additional nutrition. Magnesium sulphate serves as a source of ions. Due to the presence of the above mentioned ingredients these media allow the growth of stressed and chlorine tolerant bacteria present in treated waters.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eYellow coloured, clear solution in tubes\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 0.312% w\/v aqueous solution at 25°C. pH : 7.2±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.00-7.40\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCultural Response\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eM1687: Cultural characteristics observed *by using standard ATCC cultures after an incubation at 35-37°C for 24-72 hours.\u003c\/p\u003e\n\u003cp class=\"note\"\u003e(*-In case of water samples from fields it is suggested to incubate further for upto 7 days to ascertain the absence of organisms)\u003c\/p\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eCandida albicans\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 10231\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEnterococcus faecalis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 29212\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eEnteritidis ATCC 13076\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eTyphi ATCC 6539\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eReasoner and Geldreich, 1985, Appl. Environ. Microbiol., 49:1.\u003c\/li\u003e\n\u003cli\u003eStark and McCoy. 1938. Zentralbl. Bacteriol. Parasitenkd. Infectionskr. Hyg. Abt.2 98 : 201\u003c\/li\u003e\n\u003cli\u003eCollins and Willoughby, 1962, Arch. Microbiol., 43:294.\u003c\/li\u003e\n\u003cli\u003eGreenberg A. E., Trussell R. R. and Clesceri L. S. (Eds.), 1985, Standard Methods for the Examination of Water and Wastewater, 16th ed., APHA, Washington, DC\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himedia","offers":[{"title":"Default Title","offer_id":48363145986267,"sku":"BP-5252-HIM-HIM-DEF","price":6299.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m1687-500g_1.png?v=1773489288","url":"https:\/\/zayyuconnect.com\/ar\/products\/himedia-r2a-broth-m1687-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}