{"product_id":"himedia-sabouraud-dextrose-agar-granulated-gm063-500g","title":"HiMedia - Sabouraud Dextrose Agar, Granulated (GM063-500G)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eFor cultivation of yeasts, moulds and aciduric microorganisms, SDA, SD Agar, Granulated, SDA, SD Agar\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section intended-use\"\u003e\n\u003ch3\u003eIntended Use\u003c\/h3\u003e\n\u003cp\u003eRecommended for the cultivation of yeasts, moulds and aciduric microorganisms from clinical and non-clinical samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section composition\"\u003e\n\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDextrose (Glucose)\u003c\/td\u003e\n\u003ctd\u003e40.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMycological, peptone\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH (at 25°C)\u003c\/td\u003e\n\u003ctd\u003e5.6±0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"note\"\u003eFormula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section directions\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 65.0 gram in 1000 ml purified\/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section principle_and_interpretation\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eSabouraud Dextrose Agar is Carlier's modification (1) of the formulation described by is a modification of Sabouraud Dextrose Agar which is described by Sabouraud (2) for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections. This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (3,4). This medium is recommended by ISO 11133 for recovery of fungi and as a reference medium for other selective media for fungi (5)\u003c\/p\u003e\n\u003cp\u003eMycological Peptone provides nitrogenous compounds. Dextrose provides an energy source. High dextrose concentration and low pH favors fungal growth and inhibits contaminating bacteria from test samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section type_of_specimen\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical samples: skin scrapings, Food samples; Cosmetics.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section specimen_collection_and_handling\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (3-4).\u003c\/p\u003e\n\u003cp\u003eFor food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines(5-9).\u003c\/p\u003e\n\u003cp\u003eAfter use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section warning_and_precautions\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves\/ protective clothing\/eye protection\/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section limitations\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eFor heavily contaminated samples, the plate must be supplemented with inhibitory agents for inhibiting bacterial growth with lower pH.\u003c\/li\u003e\n\u003cli\u003eSome pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet\u003c\/li\u003e\n\u003cli\u003eFurther biochemical tests should be carried out for confirmation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section performance_and_evaluation\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section quality_control\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eCream to yellow coloured granular media.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eLight yellow to amber coloured clear to slightly opalescent gel forms in Petri plates.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eReaction of 6.5% w\/v aqueous solution at 25°C (after sterilization). pH: 5.6±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e5.40-5.80\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section growth_promoting_properties\"\u003e\n\u003ch3\u003eGrowth Promoting Properties\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eCultural Response:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCultural response was observed after an incubation at 20-25°C for upto 5 days. Recovery is considered as 100% on previously approved and validated batch of Sabouraud Dextrose Agar\u003c\/p\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCandida albicans ATCC 10231 (00054*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eLuxuriant (white colonies)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e#Aspergillus brasiliensis ATCC 16404 (00053*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCandida albicans ATCC 2091 (00055*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSaccharomyces cerevisiae ATCC 9763 (00058*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMucor racemosus ATCC 42647 (00181)*\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEscherichia coli ATCC 8739 (00012*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEscherichia coli ATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e$Lactobacillus paracasei ATCC 334\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTrichophyton rubrum ATCC 28191\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"note\"\u003eKey: (*) - Corresponding WDCM numbers.\u003cbr\u003e(#) - Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eAspergillus niger\u003c\/i\u003e\u003cbr\u003e($) - Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eLactobacillus casei\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section storage_and_shelf_life\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section disposal\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section references\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eCarlier G. I. M., 1948, Brit. J. Derm. Syph., 60:61.\u003c\/li\u003e\n\u003cli\u003eSabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003cli\u003eMicrobiology of food,animal feeding stuffs and water- Preparation, production,storage and performance testing of culture media, EN ISO 11133:2014\/Amd. 2:2020 (E).\u003c\/li\u003e\n\u003cli\u003eBacteriological Analytical Manual, 8th Edition, Revision A, 1998. AOAC, Washington D.C.\u003c\/li\u003e\n\u003cli\u003eAmerican Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.\u003c\/li\u003e\n\u003cli\u003eSalfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eWehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/GM063.pdf\" target=\"_blank\" title=\"Sabouraud Dextrose Agar, Granulated\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"Default Title","offer_id":48301372080347,"sku":"BP-5230-HIM-HIM-DEF","price":5399.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/gm063-500g.png?v=1772003181","url":"https:\/\/zayyuconnect.com\/ar\/products\/himedia-sabouraud-dextrose-agar-granulated-gm063-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}