{"product_id":"himedia-bhi-lq003a-10x70ml","title":"HiMedia - BHI (LQ003A-10X70ML)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cp\u003eThis liquid medium is a qualitative test recommended for detection of microorganisms in blood. . The medium contains Brain Heart infusion Broth (BHI) , which is  highly enriched and supports the growth of fastidious organisms.  It contains 70 ml for adult use. \u003c\/p\u003e\n\u003cp\u003eIntended Use\u003cbr\u003eRecommended as a qualitative test for detection of microorganisms in blood. Sterile, in glass bottles.\u003c\/p\u003e\n\u003cp\u003eComposition**\u003cbr\u003eIngredients    g\/L\u003cbr\u003eHM infusion powder #    12.500\u003cbr\u003eBHI powder    5.000\u003cbr\u003eProteose peptone    10.000\u003cbr\u003eDextrose (Glucose)    2.000\u003cbr\u003eSodium chloride    5.000\u003cbr\u003eDisodium phosphate    2.500\u003cbr\u003eFinal pH (at 25°C)    7.4±0.2\u003cbr\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003cp\u003e# Equivalent to Calf brain infusion from\u003c\/p\u003e\n\u003cp\u003eDirections\u003cbr\u003eLabel the ready to use blood culture bottle. Remove the Aluminium foil cap. Disinfect the part of the rubber stopper which is now exposed. Draw patient's blood with the sterile or disposable needle and syringe as explained in specimen collection and disposable column. Transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the needle and injecting the blood. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at 35±2°C for 18-24 hours and further for seven days.\u003c\/p\u003e\n\u003cp\u003ePrinciple And Interpretation\u003cbr\u003eBHI Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds (2,3). This medium is nutritious and well buffered to support the growth of wide variety of organisms (3,4,5). With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum (6) and other fungi. Proteose peptone, HM infusion powder and BHI powder serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.\u003c\/p\u003e\n\u003cp\u003eType of specimen\u003cbr\u003eClinical samples: Blood\u003c\/p\u003e\n\u003cp\u003eSpecimen Collection and Handling\u003cbr\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (7,8). After use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003cp\u003eWarning and Precautions\u003cbr\u003eIn Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves\/ protective clothing\/eye protection\/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003cp\u003eLimitations\u003cbr\u003eEach lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.\u003cbr\u003eIndividual organisms differ in their growth requirement and may show variable growth patterns on the medium.\u003cbr\u003eRecommended for Adult use only.\u003cbr\u003ePerformance and Evaluation\u003cbr\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003cp\u003eQuality Control\u003cbr\u003eAppearance\u003cbr\u003eSterile clear BHI in glass bottle.\u003c\/p\u003e\n\u003cp\u003eColour\u003cbr\u003eLight amber coloured clear solution\u003c\/p\u003e\n\u003cp\u003eQuantity of Medium\u003cbr\u003e70ml of medium in glass bottle, (For Adult use)\u003c\/p\u003e\n\u003cp\u003epH\u003cbr\u003e7.20-7.60\u003c\/p\u003e\n\u003cp\u003eSterility Check\u003cbr\u003ePasses release criteria\u003c\/p\u003e\n\u003cp\u003eCultural response\u003cbr\u003eCultural characteristics was observed after incubation at 35-37°C for 24-48 hours.\u003c\/p\u003e\n\u003cp\u003eOrganism    Inoculum\u003cbr\u003e(CFU)    Growth\u003cbr\u003eNeisseria meningitidis ATCC 13090    50-100    good-luxuriant\u003cbr\u003eStreptococcus pneumoniae ATCC 6303    50-100    good-luxuriant\u003cbr\u003eStreptococcus pyogenes ATCC 19615    50-100    good-luxuriant\u003cbr\u003eCandida albicans ATCC 10231 (00054*)    50-100    good-luxuriant\u003cbr\u003eStaphylococcus aureus subsp. aureus ATCC 25923 (00034*)    50-100    good-luxuriant\u003cbr\u003eEnterococcus faecalis ATCC 29212 (00087*)    50-100    good-luxuriant\u003cbr\u003eEscherichia coli ATCC 25922 (00013*)    50-100    good-luxuriant\u003cbr\u003eShigella flexneri ATCC 12022 (00126*)    50-100    good-luxuriant\u003cbr\u003eKey: (*) Corresponding WDCM numbers.\u003c\/p\u003e\n\u003cp\u003eStorage and Shelf Life\u003cbr\u003eStore between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003cp\u003eDisposal\u003cbr\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/LQ003A.pdf\" title=\"BHI\"\u003e\u003cstrong data-start=\"991\" data-end=\"1022\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"Default Title","offer_id":48319622250715,"sku":"BP-5234-HIM-HIM-DEF","price":2999.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/lq003a_2.jpg?v=1772608715","url":"https:\/\/zayyuconnect.com\/de\/products\/himedia-bhi-lq003a-10x70ml","provider":"Zayyu Connect","version":"1.0","type":"link"}