{"product_id":"himedia-blood-agar-base-w-low-ph-m089-500g","title":"HiMedia - Blood Agar Base w\/ low pH (M089-500G)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eAs a base to which blood may be added for use in the isolation and cultivation of fastidious pathogenic microorganisms like Neisseria, Streptococci etc from clinical samples.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended use\u003c\/h3\u003e\n\u003cp\u003eRecommended as a base to which blood may be added for use in the isolation and cultivation of fastidious pathogenic microorganisms like\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eNeisseria\u003c\/i\u003e,\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eStreptococci\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eetc from clinical samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition**\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHM infusion B from 500g #\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptose\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C)\u003c\/b\u003e: 6.8±0.2\u003c\/p\u003e\n\u003cp\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003cp\u003e#.- Equivalent to Beef heart, infusion from\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 40.00 grams in 1000 ml purified\/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 5% v\/v sterile defibrinated blood. Mix well and pour into sterile Petri plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eBlood Agar Base is a highly nutritious medium generally used as a basal medium for preparing blood agar by supplementation with blood. It can also be used as general-purpose media without the addition of blood. If the culture medium is to be used without addition of blood, the pH should be adjusted to 7.2 to 7.4, since most bacteria can grow better in a slightly alkaline medium. The low pH of Blood Agar Base w\/ low pH (pH 6.8) stabilizes the red blood corpuscles and favors the formation of clear zone of haemolysis (1). Also it is advantageous for cultivation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eStreptococci\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePneumococci\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eBlood Agar Base media can be used with added phenolphthalein phosphate (2) for the detection of phosphate producing staphylococci, with added salt and agar for assessment of surface contamination on equipment and pig carcass (3) and to determine salinity range of marine\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eFlavobacteria\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e(4). It can also be used for preparation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella Typhi\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eantigens (5).\u003c\/p\u003e\n\u003cp\u003eHM infusion B from and tryptose provides carbon, nitrogen, amino acids and vitamins. Sodium chloride helps in maintaining the osmotic equilibrium of the medium. Addition of blood makes the medium more nutritious by providing additional growth factors required by fastidious organisms. It also helps in visualizing the haemolytic reactions. However, haemolytic reactions depend on the animal blood used. Sheep blood gives best results for Group A\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eStreptococci\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e(6). But sheep blood fails to support growth of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eHaemophilus haemolyticus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esince sheep blood is deficient in pyridine nucleotides. However when horse blood is used\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eH. haemolyticus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ecolonies produce haemolysis and mimic\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eStreptococcus pyogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e(7).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical material : faeces, pus, sputum\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (8,9).\u003c\/p\u003e\n\u003cp\u003eAfter use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves\/ protective clothing\/eye protection\/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eAddition of sheep blood is recommended to detect haemolysis. This medium does not support the growth of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eH.haemolyticus\u003c\/i\u003e.\u003c\/li\u003e\n\u003cli\u003eAddition of Horse blood or rabbit blood to base medium supports growth of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eH.haemolyticus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ebut resemble beta-haemolytic\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eStreptococci\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand hence must be confirmed.\u003c\/li\u003e\n\u003cli\u003eHaemolytic pattern varies with the source of blood used.\u003c\/li\u003e\n\u003cli\u003eFurther biochemical and serological tests must be performed for confirmation.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eBasal medium: Light amber coloured clear to slightly opalescent gel. After addition of 5-7% v\/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 4.0% w\/v aqueous solution at 25°C. pH : 6.8±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e6.60-7.00\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCultural Response\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCultural characteristics observed with added 5% v\/v sterile defibrinated blood, after an incubation at 35-37°C for 18-48 hours.\u003c\/p\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth (w\/o blood)\u003c\/th\u003e\n\u003cth\u003eRecovery (w\/o blood)\u003c\/th\u003e\n\u003cth\u003eGrowth (with blood)\u003c\/th\u003e\n\u003cth\u003eRecovery (with blood)\u003c\/th\u003e\n\u003cth\u003eHaemolysis\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eNeisseria meningitidis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13090\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair-good\u003c\/td\u003e\n\u003ctd\u003e40-50%\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003ctd\u003enone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eaureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25923 (00034*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood\u003c\/td\u003e\n\u003ctd\u003e50-70%\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003ctd\u003ebeta\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus epidermidis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 12228 (00036*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood\u003c\/td\u003e\n\u003ctd\u003e50-70%\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003ctd\u003enone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStreptococcus pneumoniae\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 6303\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair-good\u003c\/td\u003e\n\u003ctd\u003e40-50%\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003ctd\u003ealpha\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStreptococcus pyogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 19615\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair-good\u003c\/td\u003e\n\u003ctd\u003e40-50%\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003ctd\u003ebeta\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003eKey: * - Corresponding WDCM numbers\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eNorton J. F., 1932, J. Lab. Clin. Med., 17:558-565.\u003c\/li\u003e\n\u003cli\u003eNoble W. C., 1962, J. Clin, Pathol., 15:552.\u003c\/li\u003e\n\u003cli\u003eHansen N. H., 1962, J. Appl. Bacteriol., 25:46.\u003c\/li\u003e\n\u003cli\u003eHayes P. R., 1963, J. Gen. Microbiol., 30:1.\u003c\/li\u003e\n\u003cli\u003eSchuber J. H., Edwards P. R. and Ramsere C. H., 1959, J. Bacteriol., 77:648.\u003c\/li\u003e\n\u003cli\u003eSnavely J. G. and Brahier J., 1960, Am. J. Clin. Pathol., 33:511.\u003c\/li\u003e\n\u003cli\u003eMurray P. R., Baron J. H., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., W.(2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M089.pdf\" target=\"_blank\" title=\"Blood Agar Base w\/ low pH\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"Default Title","offer_id":48323996549339,"sku":"BP-5245-HIM-HIM-DEF","price":5299.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m089-500g.png?v=1772705306","url":"https:\/\/zayyuconnect.com\/de\/products\/himedia-blood-agar-base-w-low-ph-m089-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}