{"product_id":"himedia-emb-agar-levine-m022","title":"HiMedia - EMB Agar, Levine (M022)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eRecommended for the isolation, enumeration and differentiation of members of Enterobacteriaceae from clinical and non clinical samples.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended Use\u003c\/h3\u003e\n\u003cp\u003eRecommended for the isolation, enumeration and differentiation of members of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacteriaceae\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003efrom clinical and non clinical samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg \/ L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium hydrogen phosphate\u003c\/td\u003e\n\u003ctd\u003e2.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLactose\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEosin - Y\u003c\/td\u003e\n\u003ctd\u003e0.400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMethylene blue\u003c\/td\u003e\n\u003ctd\u003e0.065\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C):\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.1±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 37.46 grams in 1000 ml purified\/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.\u003cspan\u003e \u003c\/span\u003e\u003cb\u003eAVOID OVERHEATING.\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCool to 50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of the medium.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003ePrecaution:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eStore the medium away from light to avoid photo-oxidation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eLevine EMB Agar was developed by Levine (1,2) and is used for the differentiation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eKlebsiella aerogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand also for the rapid identification of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eCandida albicans\u003c\/i\u003e. This medium is recommended for the detection, enumeration and differentiation of members of the coliform group by American Public Health Association (3,4,5). Weld (6,7) proposed the use of Levine EMB Agar, with added Chlortetracycline hydrochloride, for the rapid identification of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eCandida albicans\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ein clinical specimens. A positive identification of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eCandida albicans\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ecan be made after 24-48 hours incubation at 35-37°C in 10% carbon dioxide atmosphere, from specimens such as faeces, oral and vaginal secretions and nail or skin scraping etc. However, the typical appearance is variable.\u003c\/p\u003e\n\u003cp\u003eEosin Y and methylene blue make the medium slightly selective and inhibit certain gram-positive bacteria. These dyes serve as differential indicators in response to the fermentation of carbohydrates. This helps to differentiate between lactose-fermenters and non-fermenters in EMB Agar, Levine. The ratio of eosin-methylene blue is adjusted to approximately 6:1. Coliforms produce purplish black colonies due to uptake of methylene blue-eosin dye complex, when the pH drops. The dye complex is absorbed into the colony. Non-fermenters probably raise the pH of surrounding medium by oxidative de-amination of protein, which solubilizes the methylene blue-eosin complex resulting in formation of colourless colonies. Peptone serves as source of carbon, nitrogen, long chain amino acids, vitamins and other essential growth nutrients. Lactose serves as the source of energy by being the fermentable carbohydrate. Eosin-Y and methylene blue serve as differential indicators. Phosphate buffers the medium.\u003c\/p\u003e\n\u003cp\u003eThe test sample can be directly streaked on the medium plates. Inoculated plates should be incubated, protected from light. However standard procedures should be followed to obtain isolated colonies. A non-selective medium should be inoculated in conjunction with EMB Agar. Confirmatory tests should be further carried out for identification of isolated colonies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical samples - urine, faeces, oral and vaginal secretions and nail or skin scraping, Foodstuffs; Water samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (8,9).\u003c\/p\u003e\n\u003cp\u003eFor food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (5,10).\u003c\/p\u003e\n\u003cp\u003eFor water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (3).\u003c\/p\u003e\n\u003cp\u003eAfter use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves\/protective clothing\/eye protection\/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eA non-selective medium should be inoculated in conjunction with EMB Agar.\u003c\/li\u003e\n\u003cli\u003eConfirmatory tests should be further carried out for identification of isolated colonies.\u003c\/li\u003e\n\u003cli\u003eSome strains of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies do not grow in the presence of eosin and methylene blue.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eLight pink to purple homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReddish purple coloured, opalescent gel with greenish cast and finely dispersed precipitate forms in Petri plates\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 3.75% w\/v aqueous solution at 25°C. pH: 7.1±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e6.90-7.30\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCultural Response:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCultural characteristics observed after an incubation at 35-37°C for 24-48 hours.\u003c\/p\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003cth\u003eColour of Colony\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eCandida albicans\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 10231 (00054*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant (incubated in 10% carbon dioxide)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e#\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eKlebsiella aerogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13048 (00175*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood\u003c\/td\u003e\n\u003ctd\u003e40-50%\u003c\/td\u003e\n\u003ctd\u003epink-red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003eblue-black with metallic sheen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEnterococcus faecalis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 29212 (00087*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003enone-poor\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=10%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 27853 (00025*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eaureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 6538 (00032*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003enone-poor\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=10%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e^\u003ci\u003ePseudomonas paraeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 9027 (00026*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Typhimurium\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 14028 (00031*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSaccharomyces cerevisiae\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 9763\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003enone-poor\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=10%\u003c\/td\u003e\n\u003ctd\u003ecream\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eaureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25923 (00058*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003enone-poor\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=10%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 8739 (00012*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003eblue-black with green metallic sheen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eKey:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(*) Corresponding WDCM numbers.\u003c\/p\u003e\n\u003cp\u003e(#) Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacter aerogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e^ Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eLevine M., 1918, J. Infect. Dis., 23:43.\u003c\/li\u003e\n\u003cli\u003eLevine M., 1921, Bull. 62, Iowa State College Engr. Exp. Station.\u003c\/li\u003e\n\u003cli\u003eLipps WC, Braun-Howland EB, Baxter TE,eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.\u003c\/li\u003e\n\u003cli\u003eMarshall R. (Ed.), 1992, Standard Methods for the Examination of Dairy, Products, 16th ed., APHA Inc., New York.\u003c\/li\u003e\n\u003cli\u003eSalfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eWeld J. T., 1952, Arch. Dermat. Syph., 66:691.\u003c\/li\u003e\n\u003cli\u003eWeld J. T., 1953, Arch. Dermat. Syph., 67(5):433.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003cli\u003eAmerican Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M022.pdf\" title=\"EMB Agar, Levine\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong data-start=\"1311\" data-end=\"1320\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"100G","offer_id":48241712038107,"sku":"BP-5216-HIM-HIM-100","price":1099.0,"currency_code":"INR","in_stock":true},{"title":"500G","offer_id":48241712070875,"sku":"BP-5217-HIM-HIM-500","price":4649.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m022-100g.png?v=1770708496","url":"https:\/\/zayyuconnect.com\/de\/products\/himedia-emb-agar-levine-m022","provider":"Zayyu Connect","version":"1.0","type":"link"}