{"product_id":"himedia-hicrome®-esbl-agar-base-m1829-100g","title":"HiMedia - HiCrome® ESBL Agar Base (M1829-100G).","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eFor the detection of Extended Spectrum β-Lactamase-producing organisms.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended Use\u003c\/h3\u003e\n\u003cp\u003eRecommended for selective isolation Extended-Spectrum ß-lactamase-Producing Enterobacteriaeceae.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition**\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/ L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone mix\u003c\/td\u003e\n\u003ctd\u003e12.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChromogenic mixture\u003c\/td\u003e\n\u003ctd\u003e4.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer mix\u003c\/td\u003e\n\u003ctd\u003e4.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH ( at 25°C)\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e6.8±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 40.0 gram in 1000 ml purified \/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and add rehydrated contents of two vials of AC3F Selective Supplement (FD278). Mix well and pour into sterile Petri plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eExtended-spectrum ß-lactamase (ESBL)-producing organisms are an increasing challenge for healthcare practitioners fighting healthcare-associated infections (HAIs).\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEscherichia coli\u003c\/i\u003e,\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eKlebsiella pneumoniae\u003c\/i\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eKlebsiella oxytoca\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eare the most common ESBL-producing pathogens (1). ESBL-producing organisms are generally resistant to many classes of antibiotics, including aminoglycosides and fluoroquinolones; ESBL-producing organisms are able to attack newer cephems and monobactams as well as narrow-spectrum cephalosporins and antigram-negative penicillins (1). They are associated with increased mortality and are difficult to detect and treat. The widespread use of extended-spectrum, third-generation cephalosporins, introduced in the 1980's to treat antibiotic-resistant bacteria, is believed to be a major contributor to the emergence of ESBL-producing organisms.\u003c\/p\u003e\n\u003cp\u003eHiCrome®Â® ESBL Agar Base is chromogenic screening medium for the selective isolation of ESBL producing organisms. It contains peptone mix which serves as the carbon and nitrogen sources, long chain amino acids, vitamins and other growth nutrients. Chromogenic mixture is used to differentiate the ESBL producing organisms on the basis of colour. AC3F Selective Supplement (FD278) helps in inhibition of other contaminating organisms. ESBL producing\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eE.coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003egrow as either pink or purple colonies.\u003c\/p\u003e\n\u003cp\u003eESBL producing members of the KESC group produce bluish green colonies;\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eProteus\u003c\/i\u003e,\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eMorganella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eProvidencia\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003edo not utilize any chromogen resulting in colourless to light brown colonies. This medium can be inoculated with liquid suspension equivalent to 0.5 McFarland turbidity, prepared from rectal screening swabs, faecal samples or from isolated colony. Isolated colonies should not be directly plated on to this medium, because the high level inoculum may cause false positive results. Further confirmation using biochemical identification tests is recommended.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical samples - rectal screening swabs, urine, faecal samples, etc. or from isolated colony.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3).\u003c\/p\u003e\n\u003cp\u003eAfter use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves\/protective clothing\/eye protection\/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eSome species may show poor growth due to nutritional variations.\u003c\/li\u003e\n\u003cli\u003eSlight colour variation may be observed depending upon strains.\u003c\/li\u003e\n\u003cli\u003eIsolated colonies should not be directly plated on to this medium, because the high level inoculum may cause false positive results.\u003c\/li\u003e\n\u003cli\u003eFurther confirmation using biochemical identification tests is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eYellow coloured opalescent gel forms in Petri plates\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 4.0% w\/v aqueous solution at 25°C. pH : 6.8±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e6.60-7.00\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCultural Response\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCultural characteristics observed after an incubation at 35-37°C for 24 hours with added AC3F Selective Supplement (FD278).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003cth\u003eColour of Colony\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eNCTC 13351\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003epink to purple\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eKlebsiella pneumoniae\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 700603\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ebluish green\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEnterobacter cloacae\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 23355\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10⁴\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eCitrobacter freundii\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 8090\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10⁴\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eCandida albicans\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 10231 (00054*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10⁴\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"note\"\u003eKey : (*) Corresponding WDCM numbers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2, 3).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eJournal of Clinical Microbiology, February 2007, Page 501-505, Vol. 45, No. 2\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015), Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M1829.pdf\" title=\"HiCrome® ESBL Agar Base\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himedia","offers":[{"title":"Default Title","offer_id":48363151982811,"sku":"BP-5253-HIM-HIM-DEF","price":3699.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m1829-100g.png?v=1773490097","url":"https:\/\/zayyuconnect.com\/de\/products\/himedia-hicrome%c2%ae-esbl-agar-base-m1829-100g","provider":"Zayyu Connect","version":"1.0","type":"link"}