{"product_id":"himedia-nutrient-agar-1-5-m087-500g","title":"HiMedia - Nutrient Agar 1.5% (M087-500G)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eA general purpose nutrient medium which can be used for cultivation of bacteriA not requiring A highly nutritious medium. The medium can also be enriched with blood, ascitic fluid or other enriching fluids.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended use\u003c\/h3\u003e\n\u003cp\u003eA general purpose nutrient medium which can be used for cultivation of bacteria not requiring a highly nutritious medium. The medium can also be enriched with blood, ascitic fluid or other enriching fluids.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition**\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHM Peptone B#\u003c\/td\u003e\n\u003ctd\u003e3.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd\u003e8.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C):\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.3±0.2\u003c\/p\u003e\n\u003cp\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003cp\u003e#- Equivalent to Beef extract\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 31.0 grams in 1000 ml purified\/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, it can be appropriately enriched with sterile blood, ascetic fluid or serum after cooling to 45-50°C. Mix well and pour into sterile Petri plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eNutrient Agar 1.5% is the modification of Nutrient Agar recommended by APHA for cultivation and maintenance of non-fastidious microorganisms (1). This medium is used as a general-purpose medium. Recently ISO Committee (2) has also recommended it with slight modification for sub cultivation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies isolated from meat and meat products.\u003c\/p\u003e\n\u003cp\u003ePeptone is the principal source of organic nitrogen while HM Peptone B provides carbohydrates, vitamins, organic nitrogen compounds and salts. Nutrient Agar 1.5% may be used for blood culturing work after the addition of sterile 5-10% v\/v defibrinated blood. Sodium chloride makes the medium isotonic preventing haemolysis of red blood corpuscles.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical samples - faeces, urine, etc; Food and dairy samples; Water samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling:\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).\u003c\/p\u003e\n\u003cp\u003eFor food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,5,6).\u003c\/p\u003e\n\u003cp\u003eFor water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (7). After use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions :\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves\/ protective clothing\/eye protection\/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations :\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eThis medium is general purpose medium and may not support the growth of fastidious organisms.\u003c\/li\u003e\n\u003cli\u003eIndividual organisms differ in their growth requirement and may show variable growth patterns on the medium.\u003c\/li\u003e\n\u003cli\u003eEach lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within expiry period when stored at the recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of Prepared medium:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eYellow coloured clear gel forms in Petri plates. With the addition of blood Cherry red coloured opaque gel forms in Petri plates.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 3.1% w\/v aqueous solution at 25°C. pH: 7.3±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e7.10-7.50\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eCultural Response\u003c\/h3\u003e\n\u003cp\u003eCultural characteristics observed after an incubation at 35-37°C for 18-24 hours.\u003c\/p\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 27853 (00025*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eaureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25923 (00034*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eKey:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e(*) Corresponding WDCM numbers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eAmerican Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.\u003c\/li\u003e\n\u003cli\u003eWater quality: Enumeration of E.coli and total coliforms, Part 1. International Organization for Standardization (ISO), 2014, Draft ISO\/DIS 9308-1.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003cli\u003eSalfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eWehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eLipps WC, Braun-Howland EB, Baxter TE, eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M087.pdf\" title=\"Nutrient Agar 1.5%\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"Default Title","offer_id":48247297310939,"sku":"BP-5224-HIM-HIM-DEF","price":5399.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m087-500g.png?v=1770811905","url":"https:\/\/zayyuconnect.com\/de\/products\/himedia-nutrient-agar-1-5-m087-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}