{"product_id":"himedia-cetrimide-agar-mh024-500g","title":"HiMedia - Cetrimide Agar (MH024-500G).","description":"\u003cdiv class=\"attr-info\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eRecommended as a selective medium used for the isolation of Pseudomonas aeruginosa from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology. of USP\/EP\/BP\/JP\/IP\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section intended-use\"\u003e\n\u003ch3\u003eIntended use\u003c\/h3\u003e\n\u003cp\u003eRecommended for the selective isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003efrom pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP\/EP\/BP\/JP.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section composition\"\u003e\n\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGelatin peptone #\u003c\/td\u003e\n\u003ctd\u003e20.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium chloride\u003c\/td\u003e\n\u003ctd\u003e1.400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium sulphate\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCetrimide\u003c\/td\u003e\n\u003ctd\u003e0.300\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e13.600\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH after sterilization (at 25°C)\u003c\/td\u003e\n\u003ctd\u003e7.2±0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003cp class=\"note\"\u003e# Pancreatic digest of gelatin\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section directions\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 45.3 grams in 1000 ml purified\/distilled water containing 10 ml glycerin\/glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes or as per validated cycle. Cool to 45-50°C. Mix well and pour into sterile Petri plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section principle_and_interpretation\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eCetrimide Agar was described by King et al (1). This media formulation is in accordance with the harmonized method of USP\/EP\/BP\/JP (2-5). It is used as a selective medium for the isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003efrom pharmaceutical products. This medium is also used for microbial limit testing for non- sterile products. Lowburry first reported the use of cetrimide as an agent for selective isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e(6). This medium is also used for determining the ability of an organism to produce fluorescein and pyocyanin. Cetrimide (N-acetyl-N,N,N- trimethylammonium bromide) is incorporated in the medium to inhibit bacteria other than\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e. This compound a cationic detergent acts as a quaternary ammonium compound, which causes nitrogen and phosphorus to be released from bacterial cells other than\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e. Magnesium chloride and potassium sulphate incorporated in the medium enhances the production of pigment pyocyanin, which is a blue-green pigment, diffusing into the medium. This improves detection of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eon this medium. Presence of magnesium ions can also neutralizes EDTA, if present in the sample. Gelatin peptone provides the essential nutrients for growth of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas\u003c\/i\u003e, while glycerin serves as slow and continuous carbon source for the growing cell. For the isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e, plates of Cetrimide Agar should be inoculated from non-selective medium such as Soybean Casein Digest Medium (MH011). If the count is high the test sample can be directly inoculated onto this medium.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ecolonies may appear pigmented greenish (under uv light also).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section type_of_specimen\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003ePharmaceutical samples\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section specimen_collection_and_handling\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines (2-5). After use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section warning_and_precautions\"\u003e\n\u003ch3\u003eWarning and Precautions:\u003c\/h3\u003e\n\u003cp\u003eRead the label before opening the container. Wear protective gloves\/protective clothing\/eye protection\/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section limitations\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section performance_and_evaluation\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section quality_control\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003cbr\u003eCream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling\u003c\/b\u003e\u003cbr\u003eFirm, comparable with 1.36% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003cbr\u003eLight amber coloured opalescent gel with a slight precipitate forms in Petri plates\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003cbr\u003e7.00-7.40\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section growth_promotion_test\"\u003e\n\u003ch3\u003eGrowth Promotion Test\u003c\/h3\u003e\n\u003cp\u003eGrowth Promotion is carried out in accordance with the harmonized method of USP\/EP\/BP\/JP. Cultural response was observed after an incubation at 30-35°C for specified time. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section growth_promoting_properties\"\u003e\n\u003ch3\u003eGrowth promoting properties\u003c\/h3\u003e\n\u003cp\u003eGrowth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating \u0026lt;=100 cfu (at 30-35°C for \u0026lt;=18 hours).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section inhibitory_properties\"\u003e\n\u003ch3\u003eInhibitory properties\u003c\/h3\u003e\n\u003cp\u003eNo growth of the test microorganism occurs for the specified temp for not less than longest period of time specified inoculating \u0026gt;=100 cfu (at least 100 cfu) (at 30-35°C for \u0026gt;= 72 hours).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section cultural_response\"\u003e\n\u003ch3\u003eCultural Response\u003c\/h3\u003e\n\u003cp\u003eCultural characteristics observed after incubation at 30-35 °C for 18-72 hours. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.\u003c\/p\u003e\n\u003cdiv class=\"scroll-table\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eObserved Lot value (CFU)\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003cth\u003eIncubation temperature\u003c\/th\u003e\n\u003cth\u003eIncubation period\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth colspan=\"7\"\u003eGrowth promoting\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e^\u003ci\u003ePseudomonas paraeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 9027 (00026*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e25-100\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=18 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth colspan=\"7\"\u003eInhibitory\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 8739\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e3\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth colspan=\"7\"\u003eAdditional Microbiological testing\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 27853(00025*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e25-100\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e18-24 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25668 (00114*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003eluxuriant\u003c\/td\u003e\n\u003ctd\u003e25-100\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e18-24 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStenotrophomonas maltophila\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13637\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e3\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e3\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp. aureus ATCC 6538 (00032*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e3\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eStaphylococcus aureus\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003esubsp. aureus ATCC 25923 (00034*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e3\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Typhimurium\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 14028 (00031*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e3\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eProteus mirabilis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 29906 (00023*)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=10\u003csup\u003e3\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003einhibited\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0%\u003c\/td\u003e\n\u003ctd\u003e30-35 °C\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"note\"\u003eKey: (*) Corresponding WDCM numbers, ^ Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section storage_and_shelf_life\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section disposal\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/MH024.pdf\" target=\"_blank\" title=\"Cetrimide Agar\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himedia","offers":[{"title":"Default Title","offer_id":48765151281371,"sku":"BP-5330-HIM-HIM-DEF","price":5599.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/mh024-500g_1.png?v=1778652208","url":"https:\/\/zayyuconnect.com\/products\/himedia-cetrimide-agar-mh024-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}