{"product_id":"himedia-luria-bertani-hiveg®-broth-miller-miller-luria-bertani-hiveg®-broth-mv1245-500g","title":"HiMedia - Luria Bertani HiVeg® Broth, Miller (Miller Luria Bertani HiVeg® Broth) (MV1245-500G)","description":"\u003cp\u003eLuria Bertani HiVeg® Broth, Miller is used for cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.\u003c\/p\u003e\n\u003cp\u003eIntended Use\u003cbr\u003eLuria Bertani HiVeg® Broth, Miller is used for cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.\u003c\/p\u003e\n\u003cp\u003eComposition\u003cbr\u003eIngredients    Gms \/ Litre\u003cbr\u003eHiVeg® hydrolysate    10.000\u003cbr\u003eYeast extract    5.000\u003cbr\u003eSodium chloride    10.000\u003cbr\u003eFinal pH (at 25°C)    7.5±0.2\u003cbr\u003eFormula adjusted, standardized to suit performance parameters.\u003c\/p\u003e\n\u003cp\u003eDirections\u003cbr\u003eSuspend 25 grams in 1000 ml distilled water. Heat, if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.\u003c\/p\u003e\n\u003cp\u003ePrinciple and Interpretation\u003cbr\u003eLuria Bertani HiVeg® Broth, Miller is prepared by replacing casein enzymic hydrolysate with HiVeg® hydrolysate which is free of BSE\/TSE risks. Luria Bertani HiVeg® Broth, Miller (1) is slightly different with double amount of sodium chloride as compared to original media described by Lennox (2). This medium is nutritionally rich for the growth of pure cultures of recombinant strains. Strains which are generally derived from Escherichia coli K12 are deficient in Vitamin B synthesis and are unable to grow on nutritionally deficient media. HiVeg® hydrolysate provides nitrogen and carbon while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for the membrane transport and maintains osmotic equilibrium of the medium.\u003c\/p\u003e\n\u003cp\u003eQuality Control\u003cbr\u003eAppearance: Cream to yellow homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003eColour and Clarity of prepared medium: Yellow to amber coloured clear to slightly opalescent solution in tubes\u003c\/p\u003e\n\u003cp\u003eReaction: Reaction of 2.5% w\/v aqueous solution at 25°C. pH: 7.5±0.2\u003c\/p\u003e\n\u003cp\u003epH: 7.30-7.70\u003c\/p\u003e\n\u003cp\u003eCultural Response\u003cbr\u003eCultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.\u003c\/p\u003e\n\u003cp\u003eOrganism    Inoculum (CFU)    Growth\u003cbr\u003eEscherichia coli ATCC 23724    50-100    luxuriant\u003cbr\u003eEscherichia coli ATCC 25922    50-100    luxuriant\u003cbr\u003eEscherichia coli DH5 alpha MTCC 1652    50-100    luxuriant\u003cbr\u003eStorage and Shelf Life\u003cbr\u003eStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/MV1245.pdf\" target=\"_blank\" title=\"Luria Bertani HiVeg® Broth, Miller (Miller Luria Bertani HiVeg® Broth)\" rel=\"noopener\"\u003e\u003cstrong data-start=\"917\" data-end=\"928\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Himedia","offers":[{"title":"Default Title","offer_id":48391365263579,"sku":"BP-5264-HIM-HIM-DEF","price":5849.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/mv1245-500g_1.jpg?v=1773733852","url":"https:\/\/zayyuconnect.com\/products\/himedia-luria-bertani-hiveg%c2%ae-broth-miller-miller-luria-bertani-hiveg%c2%ae-broth-mv1245-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}