{"product_id":"himedia-ss-agar-salmonella-shigella-agar-m108-100g","title":"HiMedia - SS Agar (Salmonella Shigella Agar) (M108-100G)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eFor the isolation of Salmonella and some Shigella species from pathological specimens, suspected foodtuffs etc.\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended Use:\u003c\/h3\u003e\n\u003cp\u003eRecommended for the isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand some\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies from pathological specimens, suspected foodstuffs etc.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition**\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHM peptone B #\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLactose\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBile salts mixture\u003c\/td\u003e\n\u003ctd\u003e8.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium citrate\u003c\/td\u003e\n\u003ctd\u003e10.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium thiosulphate\u003c\/td\u003e\n\u003ctd\u003e8.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerric citrate\u003c\/td\u003e\n\u003ctd\u003e1.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrilliant green\u003c\/td\u003e\n\u003ctd\u003e0.00033\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeutral red\u003c\/td\u003e\n\u003ctd\u003e0.025\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C)\u003c\/b\u003e: 7.0±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003cp class=\"note\"\u003e# - Equivalent to Beef extract\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 63.02 grams in 1000 ml purified \/distilled water. Boil with frequent agitation to dissolve the medium completely.\u003cspan\u003e \u003c\/span\u003e\u003cb\u003eDO NOT AUTOCLAVE OR OVERHEAT.\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eOverheating may destroy selectivity of the medium. Cool to about 50°C. Mix and pour into sterile Petri plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eSS Agar medium is recommended as differential and selective medium for the isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies from pathological specimens (1) and suspected foodstuffs (2-5) and for microbial limit test (6). SS Agar is a moderately selective medium in which gram-positive bacteria are inhibited by bile salts, brilliant green and sodium citrate.\u003c\/p\u003e\n\u003cp\u003ePeptone, HM peptone B provides nitrogen and carbon source, long chain amino acids, vitamins and essential growth nutrients. Lactose is the fermentable carbohydrate. Brilliant green, bile salts and thiosulphate selectively inhibit gram-positive and coliform organisms. Sodium thiosulphate is reduced by certain species of enteric organisms to sulphite and H\u003csub\u003e2\u003c\/sub\u003eS gas and this reductive enzyme process is attributed by thiosulphate reductase. Production of H\u003csub\u003e2\u003c\/sub\u003eS gas is detected as an insoluble black precipitate of ferrous sulphide, formed upon reaction of H\u003csub\u003e2\u003c\/sub\u003eS with ferric ions or ferric citrate, indicated in the center of the colonies.\u003c\/p\u003e\n\u003cp\u003eThe high selectivity of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella Shigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eAgar allows the use of large inocula directly from faeces, rectal swabs or other materials suspected of containing pathogenic enteric bacilli. On fermentation of lactose by few lactose-fermenting normal intestinal flora, acid is produced which is indicated by change of colour from yellow to red by the pH indicator-neutral red. Thus these organisms grow as red pigmented colonies. Lactose non-fermenting organisms grow as translucent colourless colonies with or without black centers. Growth of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies appears as colourless colonies with black centers resulting from H\u003csub\u003e2\u003c\/sub\u003eS production.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies also grow as colourless colonies which do not produce H\u003csub\u003e2\u003c\/sub\u003eS.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eClinical: faeces, rectal swabs; Suspected food stuffs.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor clinical samples follow appropriate techniques for handling specimens as per established guidelines (7,8).\u003c\/p\u003e\n\u003cp\u003eFor food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (2-5). After use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eIn Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves\/ protective clothing\/eye protection\/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eThe medium is highly selective and may be toxic to certain\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSalmonella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eor\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies. Hence it is recommended to use to inoculate plates of less inhibitory media parallel to SS Agar, such as Hektoen Enteric Agar (M467) or Deoxycholate Citrate Agar (M065) for easier isolation of\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eShigella\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003especies (3).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eLight yellow to pink homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eGelling\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFirm, comparable with 1.5% Agar gel\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReddish orange coloured clear to slightly opalescent gel forms in Petri plates\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 6.3% w\/v aqueous solution at 25°C. pH: 7.0±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e6.80-7.20\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCultural Response\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eCultural characteristics observed after an incubation at 35-37°C for 18-24 hours.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003cth\u003eColour of colony\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e#\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eKlebsiella aerogenes\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13048 (00175*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair\u003c\/td\u003e\n\u003ctd\u003e20-30%\u003c\/td\u003e\n\u003ctd\u003ecream pink\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair\u003c\/td\u003e\n\u003ctd\u003e20-30%\u003c\/td\u003e\n\u003ctd\u003epink with bile precipitate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Choleraesuis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 12011\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless with black centre\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Typhi\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 6539\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless with black centre\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eEnterococcus faecalis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 29212 (00087*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003enone-poor\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;=10%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eProteus mirabilis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25933\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair-good\u003c\/td\u003e\n\u003ctd\u003e30-40%\u003c\/td\u003e\n\u003ctd\u003ecolourless, may have black centre\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eShigella flexneri\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 12022 (00126*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood\u003c\/td\u003e\n\u003ctd\u003e40-50%\u003c\/td\u003e\n\u003ctd\u003ecolourless\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Typhimurium\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 14028 (00031*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless with black centre\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ci\u003eSalmonella Enteritidis\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eATCC 13076 (00030*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood-luxuriant\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;=50%\u003c\/td\u003e\n\u003ctd\u003ecolourless with black centre\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eKey: *Corresponding WDCM numbers. # Formerly known as\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEnterobacter aerogenes\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eLennette and others (Eds.), 1985, Manual of Clinical Microbiology, 4th ed., ASM, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eLipps WC, Braun-Howland EB, Baxter TE, eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.\u003c\/li\u003e\n\u003cli\u003eSalfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eWehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eWilliams S., (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th Ed.,AOAC, Washington, D.C.\u003c\/li\u003e\n\u003cli\u003eThe United States Pharmacopoeia-National Formulatory (USP-NF), 2022.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M108.pdf\" title=\"SS Agar (Salmonella Shigella Agar)\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"Default Title","offer_id":48241588961499,"sku":"BP-5215-HIM-HIM-DEF","price":1199.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m108-100g.png?v=1770705411","url":"https:\/\/zayyuconnect.com\/products\/himedia-ss-agar-salmonella-shigella-agar-m108-100g","provider":"Zayyu Connect","version":"1.0","type":"link"}