{"product_id":"himedia-wl-nutrient-broth-m050-500g","title":"HiMedia - WL Nutrient Broth (M050-500G)","description":"\u003cdiv class=\"box-inner1\"\u003e\n\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"sku-block\"\u003e\n\u003cdiv itemprop=\"description\" class=\"value\"\u003eRecommended for cultivation of yeasts, moulds and bacteria encountered in brewing and industrial fermentation processes.\u003c\/div\u003e\n\u003cdiv itemprop=\"description\" class=\"value\"\u003e\u003c\/div\u003e\n\u003cdiv itemprop=\"description\" class=\"value\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eIntended Use\u003c\/h3\u003e\n\u003cp\u003eRecommended for cultivation of yeasts, moulds and bacteria encountered in brewing and industrial fermentation processes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredients\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone\u003c\/td\u003e\n\u003ctd\u003e5.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd\u003e4.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDextrose (Glucose)\u003c\/td\u003e\n\u003ctd\u003e50.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium dihydrogen phosphate\u003c\/td\u003e\n\u003ctd\u003e0.550\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium chloride\u003c\/td\u003e\n\u003ctd\u003e0.425\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCalcium chloride anhydrous\u003c\/td\u003e\n\u003ctd\u003e0.125\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium sulphate\u003c\/td\u003e\n\u003ctd\u003e0.125\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerric chloride\u003c\/td\u003e\n\u003ctd\u003e0.0025\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManganese sulphate\u003c\/td\u003e\n\u003ctd\u003e0.0025\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBromo cresol green\u003c\/td\u003e\n\u003ctd\u003e0.022\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb\u003eFinal pH (at 25°C):\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e5.5±0.2\u003c\/p\u003e\n\u003cp class=\"note\"\u003e**Formula adjusted, standardized to suit performance parameters\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDirections\u003c\/h3\u003e\n\u003cp\u003eSuspend 60.25 grams in 1000 ml purified \/ distilled water. Heat if necessary, to dissolve the medium completely. Dispense in tubes or flasks or as desired Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired to obtain a pH of 6.5, add 1% solution of sodium bicarbonate before sterilization.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePrinciple And Interpretation\u003c\/h3\u003e\n\u003cp\u003eWL (Wallerstein Laboratory) media are formulated as described by Green and Gray for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria (1, 2). Bakers yeast counts can be carried out in this medium at a pH 5.5. By adjusting the pH to 6.5, the medium can be used for obtaining counts of Baker and distillers yeast (3). If desired Durham's tubes can be added to WL Nutrient Broth to study fermentation reactions.\u003c\/p\u003e\n\u003cp\u003eYeast extract serves as a source of trace elements, vitamins and amino acids. Tryptone is used as a source of nitrogen, amino acids and carbon. Dextrose is the source of carbohydrate. Buffering of the medium is done by monopotassium phosphate. Potassium chloride, calcium chloride and ferric chloride are essential ions that help to maintain the osmotic balance. Magnesium sulphate and manganese sulphate are sources of divalent cations. Bromo cresol green is a pH indicator.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eType of specimen\u003c\/h3\u003e\n\u003cp\u003eMicroorganisms in brewery\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eSpecimen Collection and Handling\u003c\/h3\u003e\n\u003cp\u003eFor fermentation samples follow appropriate techniques for handling specimens as per established guidelines (1,2). After use, contaminated materials must be sterilized by autoclaving before discarding.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eWarning and Precautions\u003c\/h3\u003e\n\u003cp\u003eRead the label before opening the container. Wear protective gloves\/protective clothing\/eye protection\/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eFurther biochemical identification of organisms is required for confirmation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003ePerformance and Evaluation\u003c\/h3\u003e\n\u003cp\u003ePerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eQuality Control\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eAppearance:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eLigth yellow to light green homogeneous free flowing powder\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eColour and Clarity of prepared medium:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eBluish green coloured very slightly opalescent solution in tubes.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReaction:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eReaction of 6.02% w\/v aqueous solution at 25°C. pH : 5.5±0.2\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003epH:\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e5.30-5.70\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eCultural Response\u003c\/h3\u003e\n\u003cp\u003eCultural characteristics observed in tubes containing inverted Durham's tubes after an incubation at 35-37°C for 40-48 hours for bacteria and at 30-32°C upto 5 days for yeast.\u003c\/p\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eGrowth\u003c\/th\u003e\n\u003cth\u003eAcid\u003c\/th\u003e\n\u003cth\u003eGas\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATCC 25922 (00013*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair-good\u003c\/td\u003e\n\u003ctd\u003epositive reaction, yellow colour\u003c\/td\u003e\n\u003ctd\u003epositive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eLactobacillus fermentum\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATCC 9338\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003efair-good\u003c\/td\u003e\n\u003ctd\u003epositive reaction, yellow colour\u003c\/td\u003e\n\u003ctd\u003eslightly positive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eSaccharomyces cerevisiae\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATCC 9763 (00058*)\u003c\/td\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003ctd\u003egood\u003c\/td\u003e\n\u003ctd\u003epositive reaction, yellow colour\u003c\/td\u003e\n\u003ctd\u003epositive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"note\"\u003eKey: *Corresponding WDCM numbers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eStorage and Shelf Life\u003c\/h3\u003e\n\u003cp\u003eStore between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eDisposal\u003c\/h3\u003e\n\u003cp\u003eUser must ensure safe disposal by autoclaving and\/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"section\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eGreen S. R. and Gray P. P., 1950, Wallerstein Lab. Commun., 12:43\u003c\/li\u003e\n\u003cli\u003eGreen S. R. and Gray P. P., 1950, Wallerstein Lab. Commun., 13:357\u003c\/li\u003e\n\u003cli\u003eMacFaddin J. F., 1985, Media for Isolation-Cultivation- Identification- Maintenance of Medical Bacteria, Vol.1, Williams \u0026amp; Wilkins, Baltimore, Md.\u003c\/li\u003e\n\u003cli\u003eIsenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.\u003c\/li\u003e\n\u003cli\u003eJorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.himediadownloads.com\/MSDS\/M050.pdf\" target=\"_blank\" title=\"WL Nutrient Broth\" rel=\"noopener\"\u003e\u003cstrong\u003eSafety data sheet(SDS)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Himeida","offers":[{"title":"Default Title","offer_id":48323875668187,"sku":"BP-5243-HIM-HIM-DEF","price":5699.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0796\/6942\/8443\/files\/m050-500g.png?v=1772699290","url":"https:\/\/zayyuconnect.com\/products\/himedia-wl-nutrient-broth-m050-500g","provider":"Zayyu Connect","version":"1.0","type":"link"}