Luria Bertani HiVeg® Broth, Miller is used for cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.
Intended Use
Luria Bertani HiVeg® Broth, Miller is used for cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.
Composition
Ingredients Gms / Litre
HiVeg® hydrolysate 10.000
Yeast extract 5.000
Sodium chloride 10.000
Final pH (at 25°C) 7.5±0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 25 grams in 1000 ml distilled water. Heat, if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.
Principle and Interpretation
Luria Bertani HiVeg® Broth, Miller is prepared by replacing casein enzymic hydrolysate with HiVeg® hydrolysate which is free of BSE/TSE risks. Luria Bertani HiVeg® Broth, Miller (1) is slightly different with double amount of sodium chloride as compared to original media described by Lennox (2). This medium is nutritionally rich for the growth of pure cultures of recombinant strains. Strains which are generally derived from Escherichia coli K12 are deficient in Vitamin B synthesis and are unable to grow on nutritionally deficient media. HiVeg® hydrolysate provides nitrogen and carbon while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for the membrane transport and maintains osmotic equilibrium of the medium.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Yellow to amber coloured clear to slightly opalescent solution in tubes
Reaction: Reaction of 2.5% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH: 7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
Organism Inoculum (CFU) Growth
Escherichia coli ATCC 23724 50-100 luxuriant
Escherichia coli ATCC 25922 50-100 luxuriant
Escherichia coli DH5 alpha MTCC 1652 50-100 luxuriant
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Safety data sheet(SDS)
